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. 2017 Jun 29;7:279. doi: 10.3389/fcimb.2017.00279

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Copyright © 2017 Jia, Tan, Duan, Pan, Chen, Yin and Jiao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Infection kinetics in organs of mice immunized with LM4Δhly::E7. Groups of five tumor-bearing mice were intraperitoneally immunized with LM4Δhly::E7 on days 0, 7, and 14. Spleens, livers, brains, and tumors were extracted and homogenized on 1 and 3 days after each immunization. Homogenates were diluted with PBS buffer and plated on BHI agar to determine bacterial numbers. (A) CFUs/organ was calculated on 1 and 3 days after each immunization, and infection kinetics in the organs are shown. (B) LM4Δhly::E7 bacilli in the tumors were observed by electron microscopy. Scale bar 0.5 μm, white arrows, LM4Δhly::E7 bacilli. Data are expressed as the mean and SD values from three independent experiments.