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Cell death and apoptosis in the tumors of tumor-bearing mice treated with rLM. Tumors were excised 7 days after the final vaccination, and processed into single cell suspensions. Cells were then stained with Annexin-V-FITC and PI, and cell apoptosis and cell death were analyzed by flow cytometry. The rates of cell death were calculated. Data are expressed as the mean and SD values from three independent experiments. **P < 0.01, Student's t-test.
