Fig. 2.

A plasmid vector with two convergent H1 promoters driving the expression of short RNA from a single 19-bp DNA can induce efficient gene silencing, at a level that is comparable to that induced by hairpin siRNA-encoding plasmids. CTL, vector control. (A) Dose responses of pDH-RL82 within a 0.3–2.7 μg per well dose range. (B) Dose responses of pHairpin_RL82 within a 0.3–2.7 μg per well dose range. All experiments were repeated at least two times with at least three replicas in each experiment.