Fig. 6.

Diagram of the structure of randomized siRNA library constructed in pCUH. Partially randomized single-stranded library oligonucleotides were synthesized according to the common formula of 5′-AGG GGA AGA TCT AAA AAN NNN NNN NNN NNN NNN NNC TTT TTA AGC TTG GGC CGC CG-3′ where N represents wobble bases with different G/A/T/C composition as listed in Table 1. The single-stranded library oligonucleotides were annealed to CGG CGG CCC AAG and then made double stranded by a fill-in reaction with Klenow fragment. The double-stranded fragments were cleaved with BglII and HindIII and cloned into the same sites in pCUH.