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. 2017 Jun 28;2(3):e00169-17. doi: 10.1128/mSphere.00169-17

TABLE 1 .

Locations and characteristics of study sitesa

Characteristic Result(s)
Forestry
Bog		 Crystal
Bog		 North
Sparkling
Bog		 West
Sparkling
Bog		 Trout
Bog		 South
Sparkling
Bog		 Hell’s
Kitchen		 Mary
Lake
ID FB CB NS WS TB SS HK MA
Depth (m) 2 2.5 4.5 4.6 7 8 19.3 21.5
Surface area (m2) 1,300 5,600 4,700 11,900 10,100 4,400 30,000 12,000
Approx vol (m3) 867 4,667 7,050 18,247 23,567 11,733 193,000 86,000
Mixing regime Polymictic Polymictic Dimictic Polymictic Dimictic Dimictic Meromictic Meromictic
GPS coordinates 46.04777,
−89.651248		 46.007639,
−89.606341		 46.004819,
−89.705214		 46.004633,
−89.709082		 46.041140,
−89.686352		 46.041140,
−89.709082		 46.186674,
−89.702510		 46.250764,
−89.900419
Yr(s) sampled 2007 2007, 2009 2007, 2008,
2009		 2007 2005, 2007,
2008, 2009		 2007, 2008,
2009		 2007 2005, 2007,
2008, 2009
pH 4.97, 4.85 4.49, 4.41 4.69, 4.80 5.22, 5.14 4.60, 4.78 4.46, 4.94 5.81, 5.72
Dissolved inorganic
carbon (ppm)		 0.94, 1.46 0.69, 1.72 1.12, 2.31 0.76, 1.56 1.73, 4.47 1.97, 6.42 2.91, 9.70 5.54, 12.38
    SD 0.28, 1.17 0.15, 0.50 0.23, 0.72 0.17, 0.36 0.66, 54 0.24, 1.56 0.35, 1.03 5.66, 7.69
Dissolved organic
carbon (ppm)		 10.22, 8.96 15.47, 13.6 10.05, 10.40 7.26, 7.27 19.87, 20.58 12.40, 21.92 7.26, 7.33 20.63, 67.10
    SD 0.59, 0.10 4.12, 0.82 1.16, 0.96 0.43, 0.73 2.76, 1.17 0.38, 4.76 1.03, 0.12 1.91, 72.67
Total nitrogen
(ppb)		 620.57, 846.00 629.09, 809.45 737.71,
1,121.00		 813.88, 1,498 1,332.57,
3,652.38
Total phosphorus
(ppb)		 30.00, 38.86 78.00, 135.45 50.57, 53.25 48.63, 69.14 78.00, 303.50
Total dissolved
nitrogen (ppb)		 1,290.19,
490.13		 442.39, 586.56 582.5, 820.21 451.63,
1,179.21		 1,024.5,
3,220.14
Total dissolved
phosphorus
(ppb)		 84.25, 14.88 70.22, 22.67 34.5, 31.57 16.25, 18.29 71.13, 228
a

The lakes included in this time series are small, humic bog lakes in the boreal region near Minocqua, WI. They range in depth from 2 to 21.5 m and encompass a range of water column mixing frequencies (termed regimes). Dimictic lakes mix twice per year, typically in fall and spring, while polymictic lakes can mix more than twice throughout the spring, summer, and fall. Meromictic lakes have no recorded mixing events. pH was measured in 2007, while nutrient data were determined in 2008 (with the exceptions of FB, WS, and HK, measured in 2007); the two measurements were taken concurrently with the bacterial biomass collection from the same water sample. Where two values are present in a single cell, the first represents the epilimnion value and the second represents the hypolimnion value. GPS, Global Positioning System.