FIG 1 .

Subcellular localization of UUKV, HRTV, and SFTSV NSs. The subcellular localization of UUKV (A), HRTV (B), or SFTSV (C) NSs proteins was analyzed by confocal microscopy. The indicated cell lines were infected with UUKV, HRTV, and SFTSV at an MOI of 3 FFU/cell (or were mock infected), fixed at 24 h p.i., permeabilized, and probed with the appropriate NSs antibody. NSs proteins (green) and cell nuclei (blue) were visualized by confocal microscopy. (D) The subcellular localization of V5-tagged NSs proteins was also investigated. HEK293T cells were transfected with plasmids encoding UUKV, HRTV, or SFTSV NSs proteins tagged at their C terminus with a V5 tag. At 24 h posttransfection, cells were fixed, permeabilized, and probed with an anti-V5 antibody. DAPI-stained nuclei (blue) and the V5-tagged NSs proteins (red) were visualized by confocal microscopy. Scale bars indicate 20 μm.