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. 2017 Jun 29;11:44. doi: 10.3389/fninf.2017.00044

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Copyright © 2017 Rueckl, Lenzi, Moreno-Velasquez, Parthier, Schmitz, Ruediger and Johenning.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Meso-scale imaging example for using SamuROI neuronal populations in the piriform cortex of a brain slice. (A) 40× magnification GCaMP6f-fluorescence summed image of a horizontal slice from the AI95/NexCre mouse in layer 2 of the piriform cortex. (B) The summed image loaded into SamuROI in FrameView with an overlay of color-coded cellular ROIs obtained with our mask generating function. (C) The RasterView displays all ROIs that are plotted. It depicts the temporal and spatial profile of neuronal activity induced changes in GCaMP6f fluorescence during a spontaneous synchronous network event. The colored arrows correspond to the highlighted cells in (B). (D) Fluorescence traces corresponding to segments color-coded in (B) and (C). Arrows in (C) depict the starting points of the corresponding traces.