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. 2017 Jun 20;19(12):2613–2626. doi: 10.1016/j.celrep.2017.06.001

Figure 2.

Figure 2

KARs Use Local Secretory Pathway Systems

(A) Schematic of dendritic local secretory pathways in neurons, focusing on ER exit sites.

(B) Representative fixed confocal images of dendritic ERESs (using the marker mRuby-Sec23a) and SBP-EGFP-GluK2 10 min after biotin addition. White arrows in the merged panel indicate colocalization.

(C) Kymograph (Movie S2) of SBP-EGFP-GluK2 and mRuby-Sec23a up to 24 min 50 s after biotin addition, with a frame being taken every 10 s. White boxes on the merged panel show the duration of colocalization.

(D) Schematic of dendritic local secretory pathways in neurons, focusing on the Golgi.

(E) Representative fixed confocal images of SBP-EGFP-GluK2 colocalization with the Golgi marker Sialyltransferase-mCherry (Golgi) before biotin release and a line trace illustrating lack of colocalization along the line indicated in white in the merged image.

(F) Representative fixed confocal images of SBP-EGFP-GluK2 30 min after biotin-induced release with Sialyltransferase-mCherry (Golgi) and line trace quantification.

(G) Kymograph (Movie S3) of SBP-EGFP-GluK2 and GalT-mCherry (Golgi) after biotin addition up to 59 min 50 s, with a frame being taken every 10 s. White boxes on the merged panel show colocalization duration.

Scale bars, 10 μm.