Figure 4.

Differential usage of carbon substrates by C. burnetii. (A) ¹³C-Incorporation (mol%) into key metabolites related to carbon flux via glycolysis (Ala), the TCA cycle (Asp) or into cell wall derived components (DAP or GlcN). C. burnetii RSA 439 NMII wild-type was grown in ACCM-2 supplemented either with 5 mM [U-¹³C₃]serine, 5 mM [U-¹³C₆]glucose or 5 mM [U-¹³C₃]glycerol. For numerical values, see Tables S3, S4. (B) Relative carbon flux from [U-¹³C₃]serine, [U-¹³C₆]glucose or [U-¹³C₃]glycerol calculated from the ratio of ¹³C-excess values of key metabolites related to glycolysis (Ala) or the TCA cycle (Asp) in relation to ¹³C-excess values from cell wall components (DAP and GlcN). High values indicate that carbon flux is directed toward cell wall biosynthesis whereas low values indicate a high flux toward the TCA cycle.