Table 3.
Comparison of identification results of 381 isolates of Aspergillus to species level by the routine morphological identification and those by the Bruker Biotyper MALDI-TOF MS system with different score values.
Aspergillus species | No. of isolates | No. of isolates with indicated identification result/no. of isolates (%) obtained from conventional morphological methods and the Bruker Biotyper MALDI-TOF MS system with indicated ranges of score values | |||||||
---|---|---|---|---|---|---|---|---|---|
Identification to species level by conventional morphological methods (n = 367) | No. of isolates with indicated score values obtained by the Bruker Biotyper MALDI-TOF MS system | ||||||||
Correct identification | Incorrect identification | ≥2.000 (n = 115) | ≥1.700 (n = 303) | ||||||
Correct identification | Incorrect identification | Correct identification | Incorrect identification | ||||||
ASPERGILLUS SPECIES COMMONLY ENCOUNTERED (n ≥ 10) | |||||||||
A. fumigatus | 107 | 105/105 (100) | 0 | 18 /18 (100) | 0 | 98/98 (100) | 0 | ||
A. flavus | 93 | 91/91 (100) | 0 | 27/28 (96.4) | 1/28 (3.6) | 78/80 (97.5) | 2/80 (2.5) | ||
A. niger | 75 | 74/75 (98.7) | 1/75 (1.3) | 40/40 (100) | 0 | 62/62 (100) | 0 | ||
A. terreus | 43 | 42/42 (100) | 0 | 12/12 (100) | 0 | 28/28 (100) | 0 | ||
A. versicolor | 11 | 2/11 (18.2) | 9/11 (81.8) | 7/7 (100) | 0 | 9/9 (100) | 0 | ||
A. sydowii | 11 | 6/6 (100) | 0 | 0 | 1/1 (100) | 0 | 6/6 (100) | ||
A. nidulans | 10 | 9/10 (90.0) | 1/10 (10.0) | 6/6 (100) | 0 | 9/9 (100) | 0 | ||
Subtotal | 350 | 329/340 (96.8) | 11/340 (3.2) | 110/112 (98.2) | 2/112 (1.8) | 284/292 (97.3) | 8/292 (2.7) | ||
ASPERGILLUS SPECIES RARELY ENCOUNTERED (n < 10) | |||||||||
A. tubingensis | 7 | 0 | 7/7 (100) | 0 | 1/1 (100) | 0 | 5/5 (100) | ||
A. japonicus | 5 | 0 | 5/5 (100) | 0 | 0 | 0 | 0 | ||
A. nomius | 4 | 0 | 4/4 (100) | 0 | 0 | 1/1 (100) | 0 | ||
A. tamarri | 3 | 0 | 2/2 (100) | 0 | 0 | 1/1 (100) | 0 | ||
A. aculeatus | 2 | 0 | 2/2 (100) | 0 | 0 | 0 | 0 | ||
A. ustus | 2 | 0 | 0 | 1/1 (100) | 0 | 1/1 (100) | 0 | ||
Others | 8 | 0 | 7/7 (100) | 1/1 (100) | 0 | 1/3 (33.3) | 2/3 (66.7) | ||
Subtotal | 31 | 0 | 27/27 (100) | 2/3 (66.7) | 1/3 (33.3) | 4/11 (36.4) | 7/11 (63.6) | ||
Total (%) | 381 | 329/367 (89.6) | 38/367 (10.4) | 112 /115 (97.4) | 3/115 (2.6) | 288/303 (95.0) | 15/303 (5.0) |