Fig. 4.

Ginseng root, ginseng berry, and syringaresinol induce the nuclear translocation of forkhead box-O3a (FoxO3a). (A) The indicated ginseng components were added to MNT1 cells for 48 h. The cells were stained with an antiFoxO3a antibody (FoxO3a; red) and 4′,6–diamidino–2–phenylindole (DAPI) (nucleus; blue). Representative images were obtained using a confocal laser scanning microscope (LSM7, Carl Zeiss, NY, USA) and merged. (B) Cells with FoxO3a localization were counted and depicted by the means of a confocal laser scanning microscope. Data are presented as mean ± standard deviation. Scale Bar = 100 μm. Ph, phase-contrast image.