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Effects of autophagy inhibitor hydroxychloroquine (HCQ) on KG-135-mediated growth inhibition and apoptosis induction in A549 human nonsmall cell lung cancer cells. (A) After treatment with KG-135 alone or in the presence of HCQ for 72 h, the cell viability was determined using sulforhodamine B binding assay and (B) examined using phase contrast microscopy, scale bar = 100 μm. (C) The sub-G1 apoptotic fraction was analyzed using flow cytometry.
