Fig. 3.

Molecular mechanism of SF-mediated apoptotic death of C6 glioma cells. (A) C6 glioma cells were treated with either WE, NSF, or SF (200 μg/mL) for 6 h, and mRNA levels of Bcl-2, BAX, and p53 were determined by semiquantitative PCR. (B) C6 glioma cells were treated with either WE, NSF, SF (200 μg/mL), or staurosporine (5μM) for 24 h, and protein expression levels of pro-caspase-3, cleaved caspase-3, and Bcl-2 were determined by Western blot analysis. BAX, Bcl-2-associated X protein; Bcl-2, B-cell lymphoma-2; NSF, nonsaponin fraction; SF, saponin fraction; WE, water extract.–