Figure 4.

Effects of DHE on COX-2 protein and mRNA expression in U87 and U251 cells. The U87 and U251 cells were treated with various concentrations of DHE (0, 10, 20 and 30 μM) for 48 h. And the expression of COX-2 protein and mRNA in the cells were evaluated by western blot (A, B) and RT-PCR (C, D), respectively. The β-actin and GAPDH served as the protein and mRNA loading control, respectively. Quantitative analyses of the band density were normalized to each loading control. The data are presented as the mean ± SD of five independent experiments. (*P<0.05, **P<0.01 and ***P<0.001 as compared with the control group).