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. 2017 Jun 1;7(6):1285–1297.

Figure 3.

Figure 3

Overexpression of ANXA4 in HCC cells promotes proliferation in vitro. A. qRT-PCR analysis of ANXA4 expression in HCC cell lines (left), and western blot analysis of ANXA4 expression in HCC cell lines (right). B. qRT-PCR analysis of ANXA4 expression in HCC cells stably transfected with ANXA4 or control plasmids (left), and western blot analysis of ANXA4 protein expression in HCC cells stably transfected with ANXA4 or control plasmids (right). C. Cell growth was analyzed at different time points by MTT assay. D. A colony formation assay was performed in HCC cells stably transfected with ANXA4 or control plasmids (left). The results of the statistical analysis are shown on the right. *P < 0.05, **P < 0.01.