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Knockdown of ANXA4 inhibits cell proliferation in vitro. A. Western blot analysis of ANXA4 expression in four shANXA4-HCC cell lines. B. Cell growth was analyzed at different time points by MTT assay. C. A colony formation assay was performed in 4 HCC cell lines stably transfected with shANXA4 or control plasmids (shNC) (images). The results of the statistical analysis are shown in a bar graph. *P < 0.05, **P < 0.01.
