Figure 5.

ANXA4 silencing suppresses HCC tumorigenesis in vivo. (A) MHCC-LM3 cells stably transfected with shANXA4-3 were subcutaneously injected into nude mice; shNC was used as a control, and the tumors were removed from the nude mice after 4 weeks. (B) The weight of the xenograft tumors shown in (A) was quantified in (B). (C) qRT-PCR and western blot analysis of ANXA4 expression in the MHCC-LM3 cell-derived xenograft samples. (D-F) We observed the same subcutaneous tumorigenesis result in mice injected with Hep3B HCC cells stably transfected with shANXA4-3. *P < 0.05, **P < 0.01.