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. 2017 Jun 1;7(6):1285–1297.

Figure 6.

Figure 6

Ikaros directly binds to and represses the ANXA4 promoter in HCC cells. A. Identification of potential Ikaros binding sites in the ANXA4 promoter according to the PROMO and Gene Regulation databases. B. Activity of the ANXA4 promoter after transfection of Ikaros into HCC cells. C. The promoter activity of ANXA4 was detected after transfection with truncated ANXA4 promoter constructs. D. The mutated sequence is shown at the predicted Ikaros binding site on the ANXA4 promoter. E. The activities of the ANXA4 promoter and mutant promoter after transfection of HCC cells with Ikaros. F. The binding of Ikaros to the ANXA4 promoter in HCC cells was determined by chromatin immunoprecipitation, and qRT-PCR was used to analyze the ANXA4 promoter (top); agarose gel electrophoresis shows the crosslinking status (bottom). *P < 0.05, **P < 0.01.