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. 2017 Jun 1;7(6):1298–1309.

Figure 2.

Figure 2

Silencing HOTAIR inhibits thyroid cancer cell growth. A. The expression level of HOTAIR was measured by qRT-PCR in TPC-1 and FTC-133 cells transfected with siRNAs of HOTAIR (si-HOTAIR1#, si-HOTAIR2#, si-HOTAIR3#) and control. B, C. Silencing HOTAIR by si-HOTAIR3# significantly inhibited proliferation of TPC-1 and FTC-133 cells at 12 hrs., 24 hrs., and 48 hrs. Cell proliferation was detected by MTT assay. D. Cell proliferation was detected by colony-forming unit assay in TPC-1 and FTC-133 cells transfected with si-HOTAIR3# and control. E. Apoptosis was measured by Annexin V-APC/7-AAD staining in TPC-1 and FTC-133 cells transfected with si-HOTAIR3# and control. F. Tumors were collected from athymic mice injected with TPC-1 cells transfected with si-HOTAIR3# or control. G. The tumor volume was analyzed at specific times. H. The tumor weight was measured at specific times (n = 7). (*P < 0.05, **P < 0.01, ***P < 0.001).