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Bio-assay guided isolation of triterpene saponins. The bio-assay guided isolation allows the isolation and identification of biological active saponins. The raw extract is fractionated into sub-fractions, which are tested in suitable bio-assays. Bio-active sub-fractions are further purified resulting in a sub-sub-fractionation and so forth. Liquid chromatography–(tandem) mass spectrometry and high performance thin layer chromatography analyses are conducted at each point.
