(a) Free membrane fluctuation spectrum. Simulation parameters are as follows L = 800 nm, kBT = 4.3 pN·nm, B = 20 pN·nm ~ 5 kBT, η = 0.06 Poise, time step Δt = 0.5 ns, total simulation time ttotal = 1 ms, and λmin = 20 nm. A simulation snapshot showing fluctuation magnitude and morphology in physics space is plotted as an inset. (b) Entropic repulsive interaction between the fluctuating membrane and the substrate. Simulation parameters are as follows: kBT = 4.3 pN·nm, B = 20 pN·nm, η = 0.06 Poise, εw = 0.043 pN/nm, time step Δt = 0.5 ns, and total simulation time ttotal = 0.6 ms. The triad number denotes in units of nm.