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. 2017 Jun 15;9(6):2748–2759.

Figure 5.

Figure 5

DHA inhibited expression of chondrocyte hypertrophic genes. MSCs were induced by chondrogenic medium without DHA for14 d and then treated with hypertrophic medium together with or without DHA for another 7 d and 14 d. (A) Representative immunohistochemical images of Runx2 and Col10a1 from MSCs in different groups (day 21 and 28). Scale bar represents 200 μm. (B) Quantification of mean intensity of Runx2 and Col10a1 in (A). (C) Relative mRNA expression levels of Runx2 and Col10a1 from MSCs in different groups (day 17, 21 and 28). (D) Representative western blot images of Col10a1, Runx2 and GAPDH from MSCs in different groups (day 17, 21 and 28). (E) Quantification of normalized expression intensity of Col10a1 and Runx2 against GAPDH. The data in the figures represent the averages ± SD. Significant differences between the treatment and control groups are indicated in the graph with the detailed corresponding P-values from Student-Newman-Keuls post hoc tests, *(P<0.05) or **(P<0.01) or ***(P<0.001).