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IFNs up-regulate DKK1 expression but show no effects on GSK3β. A549 and Calu-3 cells were treated with or without IFNα (100 ng/ml), IFNγ (100 ng/ml) or IFNλ1 (100 ng/ml), and levels of DKK1 were measured by ELISA (A, C) at 48 hours post-treatment. Activated GSK3β level was evaluated by flow cytometry in A549 and Calu-3 cells at 48 hours upon different IFN stimulation (B, D). Data represent a minimum of three experiments and asterisks denote P<0.05 in comparison to untreated samples.
