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. 2017 Jun 15;9(6):2824–2837.

Figure 4.

Figure 4

CXCL10 regulates the EMT in HCC cells. A. Silencing CXCL10 in MHCC97H increased up-regulation of E-cadherin and down-regulation of N-cadherin, fibronectin, and vimentin. MHCC97H cells, seeded in 6-well plates, were transfected with shCXCL10 or control shRNA. After 24 h since transfection, cells were subjected to western blot analysis for measuring protein levels. B. MHCC97H cells, seeded in 6-well plates, were transfected with shCXCL10 or control shRNA. After 24 h since transfections, qRT-PCR analysis was performed to assess epithelial and mesenchymal cell marker levels. C. Western blot analysis was performed to determine the epithelial and mesenchymal cell marker expression levels in HepG2 cells transfected with CXCL10 plasmid or control vector. D. HepG2 cells were seeded in 6-well plates and then transfected with CXCL10 or control vector. qRT-PCR analysis showed that CXCL10 over-expression led to the down-regulation of epithelial cell marker (E-cadherin) and up-regulation of mesenchymal cell marker (N-cadherin, fibronectin, and vimentin) mRNA levels.