Figure 5.

CXCL10 regulates MMPs expression. A. Clustering of genes differentially expressed after CXCL10 silencing. B. Enrichment scores of differential gene expression in the CXCL10-silencing cell line. MMP expression was affected by CXCL10. C. MHCC97H cells were seeded in 6-well plates and transfected with shCXCL10 or control shRNA. After 24 h since transfection, total protein samples were subjected to western blot analysis for measuring MMP-2 and MMP-9. D. The MMP-2 and MMP-9 expression levels in the CXCL10 silencing cell line were assayed by qRT-PCR analysis. The data were presented as mean ± SD. For indicated comparisons, *P < 0.05 and **P < 0.01 compared to control MHCC97H cells. E. Control vector or CXCL10 were transfected into HepG2 cells. After 24 h since transfection, MMP-2 and MMP-9 expression in CXCL10 overexpression cell line was detected by western blot analysis. F. The mRNA levels of MMP-2 and MMP-9 in indicated HepG2 cells was measured by qRT-PCR. The data are presented as mean ± SD. For indicated comparisons, *P < 0.05 and **P < 0.01 compared to control HepG2 cells.