Figure 5.

The effects of ZEB2 on growth and metastasis of HCT116 cells in vivo. A. Representative images of histologic inspection of a mouse lung for the presence of microscopic lesions twelve weeks after tail vein injection with HCT116 cells (left). Quantification of lung microscopic nodules in the lungs of each group was shown (right). Scale bar represents 100 μm. B. As a subcutaneous tumor model, BALB/c nude mice were injected subcutaneously with HCT116 cells and followed by the determination of the tumor volume. Scale bar represents 1 cm. C. Mice bearing HCT116 tumor xenograft were sacrificed at the end of the experiment, and tumor tissues were removed for future immunohistochemistry analysis with anti-Ki67 and anti-CD31 antibodies. The data were presented as the mean ± SD. *P < 0.05, **P < 0.01 vs. sh Control group.