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. 2005 Feb 3;102(7):2596–2601. doi: 10.1073/pnas.0407836102

Fig. 5.

Fig. 5.

Sox2 inhibits myelination and enhances Schwann cell proliferation. (A) Quantitative RT-PCR after infection of Schwann cells, in the presence of forskolin (6 μM), with GFP or Sox2 adenovirus demonstrated the suppression of differentiation genes (left group), whereas immature marker genes (right group) were not affected. Immunostaining for Mpz in Schwann cells infected with GFP control (B) or Sox2 (C) adenovirus illustrated the decrease in Mpz levels in Sox2-expressing Schwann cells. Myelination in vitro using either control GFP or Sox2 lentivirus-infected Schwann cells showed many myelin basic protein-positive myelin profiles in cocultures containing GFP-expressing Schwann cells (D), but not in cocultures containing Sox2-expressing Schwann cells (E). Quantification of the myelin profiles from three independent in vitro myelination experiments is summarized in F. BrdUrd incorporation assays performed on uninfected, or GFP or Sox2 lentivirus-infected Schwann cells demonstrate similar proliferation rates in the absence of β-neuregulin (G Left). In the presence of β-neuregulin (G Right), uninfected Schwann cells (H) and GFP-expressing Schwann cells (I) showed similar proliferation rates, whereas Sox2-expressing Schwann cells (J) showed a 1.8-fold greater BrdUrd incorporation rate than did GFP-expressing Schwann cells (P = 0.035, Student's t test, data represent three independent experiments). NRG, neuregulin.