Figure 3.

AngII increased Piezo1 expression in NRVMs. A: Piezo1 mRNA levels in NRVMs treated with interleukin-6 (IL-6 10 μmol/L), hydrogen peroxide (H₂O₂ 100 μmol/L), AngII (1 μmol/L) and 10% cyclic stretching, respectively (n=5). B: Piezo1 protein was analyzed by Western blot in cultured cells treated with AngII (1 μmol/L) at the indicated time points (n=4). C: Piezo1 protein was analyzed by Western blot in cultured cells treated with AngII at indicated concentrations for 24 hours (n=4). The relative expression level of Piezo1 was calculated as a foldchange normalized by GADPH. Data are presented as mean ± SEM. *P<0.05 vs control; #P<0.05 vs AngII 24 hours. Oneway ANOVA analysis with Bonferroni test was performed among all groups.