Piezo1 was elevated by AngII through AT1 receptors-Erk1/2 pathway. A: Piezo1 protein was detected by Western blot in NRVMs with 1 hour pretreatment of losartan (AT1 blocker, 10 μmol/L) or PD123319 (AT2 blocker, 10 μmol/L) before AngII exposure (1 μmol/L) for 24 hours (n=4). B: Piezo1 protein levels were measured in NRVMs pretreated for 1 hour with U0126 (Erk1/2 inhibitor, 10 μmol/L), SB203580 (p38 MAPK inhibitor, 10 μmol/L) and SP600125 (JNK inhibitor, 10 μmol/L), followed by AngII exposure (1 μmol/L) for 24 hours (n=4). c: Evaluation of the phosphorylation of Erk1/2 during AngII infusion pretreat with losartan (10 μmol/L) or PD123319 (10 μmol/L) for 30 min (n=4). Data are presented as mean ± SEM. *P<0.05 vs control; #P<0.05 vs AngII 24 hours. Oneway ANOVA analysis with Bonferroni test was performed among all groups.