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. 2017 Jun 15;9(6):3120–3137.

Figure 2.

Figure 2

Mitochondria related genes are regulated by miR-1825. (A) Pathways altered in Adult cardiomyocytes (ACMs) transfected with miR-1825 compared to control ACMs revealed through RNA-sequencing analysis. (B) In-silico analysis indicating a direct binding site for miR-1825 on the 3’UTR of NDUFA10, confirmed with luciferase assay. (C) Protein level expression of NDUFA10 in Adult CMs transfected with miR-1825. (D) Mitochondrial number; (E) DNA synthesis post NDUFA10 gene knock-down in Adult CMs. N≥3; *P<0.05. VDAC used as mitochondrial marker; TnI used as cardiac cell marker; DAPI-nucleus. SiNDU = siNDUFA10. Scale bar = 100 µm (D), Scale bar = 100 µm (E). Control (cel-miR-67).