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. 2005 Feb 3;102(7):2396–2401. doi: 10.1073/pnas.0409861102

Fig. 3.

Fig. 3.

Rescue experiment. (a–d) Phase contrast microscopic images of WT HeLa cells (a and c) and BAC transgenic HeLa cells expressing mSNW1 (b and d)96h after transfection with esiRNA targeting hSNW1 (c and d) and firefly luciferase as negative control (a and b). (e) Effect on cell viability of esiRNA targeting hSNW1. Cells were assayed 96 h after transfection. Shown is the reduction of cell viability as determined with the WST-1 assay normalized against the negative control luc (esiRNA directed against firefly luciferase). (f–i) WT HeLa cells (f and h) and BAC transgenic HeLa cells expressing mSNW1 (g and i) were transfected with esiRNA targeting hSNW1 (h and i) and firefly luciferase as negative control (f and g) and imaged by 3D deconvolution microscopy 48 h after transfection for tubulin (green) and DNA (blue). [Scale bars: 100 μm(a–d) and 5 μm(f–i).] (j) Frequency of aberrant spindles. We counted the frequency of aberrant spindles 48 h after transfection for 50 mitotic cells per coverslip. Error bars represent the standard variation. mSNW1-BAC, HeLa cells that have stably integrated the BAC carrying mSNW1; luc, esiRNA targeting firefly luciferase; hSNW1, esiRNA targeting hSNW1.