Figure 3.

After formation of surface-modified CA as described above, centrifugation was done at 4 °C with 13,000 rpm for 15 min. The supernatant was discarded and the pellet was resuspended at 100 μL DMEM. Six microliters of samples and loading dye with 1:1 ratio were loaded into each gel well (BioRad Precast Gels 7.5%) and run through SDS PAGE at 60 V for 1 h. The resulting gel was processed through silver staining.