Fig. 2.

Cumulative rRNA gene analyses. (A) Distribution of sequences by phylogenetic group as identified with arb. Universal PCR primers (515F and 1391R) were used with environmental DNA templates from five hot springs, and resultant sequences were compiled for the assemblage. Five percent of the sequences are from one potentially new candidate bacterial division encountered in this study. (B) Distribution of archaeal sequences in three hot springs with two archaeal-specific PCR primer pairs. The majority, 77% of the sequences, are identified as crenarchaeotes. Eighteen percent fall within Euryarchaeota, and 5% fall within Korarchaeota. OPA-2, OPA-4, and OPA-Like represent environmental DNA sequences from a previous study of Obsidian Pool (16); FCG-1 represents sequences from marine/hydrothermal vent benthic archaea; and SEGMEG-1 represents sequences from deep South African mines.