Fig. 3.

Detection of HCV proteins by immunofluorescence. (A) Low-power view of cells transfected with pTHr and stained without primary antibody but with the secondary antibody. No fluorescence was seen. (B) Low-power view of cells transfected with pTHr and stained with anti-core. Multiple cells with fluorescence can be seen. (C) Low-power view of cells transfected with the control pTRE and stained with anti-core. There was no fluorescence. (D) High-power view of B. (E and F) High-power views of cells stained with anti-E2. Cells were transfected with pTRE (E) or pTHr (F). (G and H) High-power views of cells transfected with pTRE (G) and pTHr (H) and stained with anti-NS5A.