Forced-swim test 3 d after CUS. The 5HT2A/C agonist DOI or vehicle aCSF was injected bilaterally into the mPFC 15 min before forced-swim testing (NoStressVehicle, n = 7; StressVehicle, n = 7; NoStressDOI, n = 7; and StressDOI, n = 7). a, The StressVehicle group showed significantly lower latency to immobility during the forced-swim test compared with all other groups (*p < 0.05, StressVehicle vs all other groups). b, The StressVehicle group showed significantly longer duration spent in immobile behavior during the forced-swim test compared with all other groups (*p < 0.05, StressVehicle vs all other groups).