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. Author manuscript; available in PMC: 2017 Jun 29.
Published in final edited form as: J Am Chem Soc. 2015 Mar 4;137(10):3486–3489. doi: 10.1021/jacs.5b00670

Figure 1.

Figure 1

(a) Quantification of the fraction of strands that hybridize to an SNA functionalized with ∼80 complementary strands as a function of stoichiometry for oligonucleotides of several different lengths. (b) Dehybridization “melting” curves for SNAs prepared with differing numbers of hybridized 9-mer complementary strands, enumerated in the legend. Inset: the first derivative of each curve, the full width at half-maximum of which is a measure of the “sharpness” of the transition. (c) Calculated SNA binding constants as a function of the number of pre-hybridized strands. Inset: the linear van't Hoff plots from which the thermodynamic quantities have been extracted. The legend describes the values of n probed in this experiment. (d) Trends in the enthalpy (left axis, blue) and entropy (right axis, green) of SNA binding as a function of the number of pre-hybridized strands.