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(a) Comparison of binding isotherms obtained from SNAs functionalized with either ∼40 or ∼80 DNA strands. Insets: schematic illustration of the role of oligo ethylene glycol moieties (shown in green) in diluting the surface DNA density compared to the densely functionalized particles investigated previously. (b) Comparison of the binding constants of SNAs functionalized with ∼40 or ∼80 surface-bound DNA strands and prehybridized with n = 20 duplexes.
