Fig. 6.

MRCCAT1 promotes ccRCC cell metastasis by downregulating NPR3 expression. a Correlation of MRCCAT1 and NPR3 at RNA levels in 68 ccRCC tumor samples. r = −0.28, P = 0.042 by Pearson correlation analysis. b Relative expression of NPR3 at mRNA levels between Lv-shNC and Lv-shMRCCAT1 cell lines. c Relative expression of NPR3 at mRNA levels between negative control and MRCCAT1 overexpression cell lines. d Western blot analysis of NPR3 upon MRCCAT1 knockdown. β-actin was used as an internal control. e Western blot analysis of NPR3 upon MRCCAT1 knockdown in lung metastatic tumor of nude mice. β-actin was used as an internal control. f CCK-8 assay in MRCCAT1 overexpressed and control 786-O cells transfected with pLVX-NPR3 at indicated times. g CCK-8 assay in MRCCAT1 overexpressed and control Caki-1 cells transfected with pLVX -NPR3 at indicated times. h Left: Transwell assays in MRCCAT1 overexpressed and control 786-O cells transfected with pLVX -NPR3. Scale bar = 200 μm. Right: The statistical graph indicates the number of cells from 8 random high power fields counted from three independent experiments. i Left: Transwell assays in MRCCAT1 overexpressed and control Caki-1 cells transfected with pLVX -NPR3. Scale bar = 100 μm. Right: The statistical graph indicates the number of cells averaged from 8 random high power fields. The results are presented as the mean ± SD from three independent experiments; *P < 0.05, **P < 0.01 by Student’s t-test