Effect of C2-ceramide, PACAP, or both on tubulin distribution and expression in cerebellar granule cells. (A–D) Immunohistochemical micrographs illustrating the distribution of tubulin in control conditions (A)orinthe presence of 20 μM C2-ceramide (B), 10-7 M PACAP (C), or C2-ceramide plus PACAP (D). Arrows show association of tubulin with neuritic processes. Scale bar, 10 μm. (E and F) Western blot analysis showing the absence of effect of 20 μM C2-ceramide (E) or 10-7 M PACAP (F) on tubulin expression.