Western blot analysis showing the effect of C2-ceramide, PACAP, or both on the concentration of Tau and phospho-Tau in cerebellar granule cells. (A and B) Time course of the effect of 20 μM C2-ceramide on the levels of Tau (A) and phospho-Tau (B). (C and D) Time course of the effect of 10-7 M PACAP on the levels of Tau (C) and phospho-Tau (D). (E) Effect of a 6-h incubation of cells in the absence or presence of C2-ceramide plus PACAP on the level of phospho-Tau. **, P < 0.01; ***, P < 0.001 vs. control; ###, P < 0.001 vs. C2-ceramide.