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. 2017 Jun 26;8:15908. doi: 10.1038/ncomms15908

Figure 5. Depletion of COBRA1 ameliorates BRCA1 mutation-related R-loop accumulation in T47D breast cancer cells.

Figure 5

(a) Immunoblots of BRCA1 and COBRA1 in knockdown T47D cells. α-Tubulin was used as the loading control. (b and c) Relative DRIP signal at (b) TRIB1 and (c) ELF3 loci in control and BRCA1 KD cells, with or without treatment of RNase H. The graph is an average of three individual experiments. (d and e) Pol II pausing index at (d) TRIB1 and (e) ELF3 loci in BRCA1 and/or NELFB KD T47D cells. Pol II pausing index is calculated by the ratio of total Pol II signals at TSS over gene body. The graph is an average of at least four individual experiments. (f and g) Relative DRIP signal at (f) TRIB1 and (g) ELF3 loci in BRCA1 and/or COBRA1 KD T47D cells. The graph is an average of at least six individual experiments. *P<0.05, **P<0.01 and ***P<0.001 by two-tailed t-test. Error bars represent s.e.m.