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. 2017 Jun 26;8:15908. doi: 10.1038/ncomms15908

Figure 6. Ablating COBRA1 mitigates BRCA1-associated R-loop accumulation and mammary tumorigenesis.

Figure 6

(a) Low and high (inlet) magnification images of R-loop staining in mammary ducts of 8-week-old virgin mice, with and without pretreatment of RNase H (RH). Scale bars, 5 μm (top) and 20 μm (bottom). (b) Quantitation of relative R-loop intensity in 8-week-old animals. The number of animals used in each group is: WT (n=9), BKO (n=9), BKO+RH (n=3), CKO (n=5) and DKO (n=8). *P<0.05 by two-tailed t-test. Error bars represent s.e.m. (c) Kaplan–Meier curve for tumour incidence. **P<0.01 by log-rank test. (d) Percentage of BrdU+ mouse mammary epithelial cells with 53BP1 foci from 12-week-old mice. The number of animals used in each group is: WT (3), BKO (3) and DKO (3). *P<0.05 by two-tailed t-test. Error bars represent s.e.m.