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. 2005 Feb 7;102(7):2655–2660. doi: 10.1073/pnas.0409816102

Fig. 5.

Fig. 5.

Coinoculation on S. rostrata with ORS571-oac2 and purified LPS (100 μg/ml). (A) Pale-red central tissue indicative for the presence of leghemoglobin in a partial Fix+ stem nodule (17 dpi). (B) Semithin section of a stem nodule with a wild-type-like fixation zone. (C) Detail of B showing the zonation of the nodule. (D) Root nodule with wild-type fixation zone. (E) ORS571-oac2[pRS2002] inoculation without LPS (19 dpi); dotted staining from nifH expression visible in bacteria located in the plant cell cytoplasm and not in bacteria present in infection pockets and threads. (F) Detail of E. (G and H) ORS571-oac2[pRS2002] inoculation with LPS (19 dpi); extensive staining from nifH expression in bacteria throughout the infection zone (G) and nifH-expressing bacteria densely colonizing infected plant cells (H). FZ, fixation zone; IP, infection pocket; IZ, infection zone; M, nodule meristem; arrows, infection threads. (Bars: 1 mm in A, B, and D; and 250 μm in C and E–H.)