Figure 2.
GC-Imaging recordings reveal minute impurities in commercial odorant sources. Each panel shows a GC-FID recording (red trace) and a simultaneous antenna calcium imaging trace from Or42b > GCaMP6m flies (black trace). All odors were injected as headspace samples at 10−2 dilution. The yellow bar indicates the elution time for ethyl acetate (100 s). A response in Or42b at that elution time is present in several samples (left column), but other impurities were also found (see response to ethyl butyrate). All traces: N = 4-5, average ± SEM For GC-FID traces, the error is smaller than the line width. Bottom right: schematic of the experimental setup.