Table 2. Summary of the RecQ4 ARL analysis presented in Fig. 7a–e.
| WT | W613L | H615L | F617L | R618L | |
|---|---|---|---|---|---|
| % WT ATPase | 100 | 63.2 | 61.7 | 34.7 | 21.1 |
| % WT Helicase | 100 | 58.2 | 50.5 | 84.7 | 2.0 |
| KDNA (nM) | 46.9±7.3 | 79±9.6 | 57.1±4.3 | 39.7±2.1 | 137.1±10.9 |
| Efficiency (Helicase/ATPase) | 1.00 | 0.92 | 0.82 | 2.44 | 0.1 |
ARL, aromatic-rich loop; WT, wild type.
Efficiency is defined by the amount of helicase activity (% WT Helicase) per ATPase activity (% WT ATPase) for each ARL variant. Therefore, efficiency values of 1 indicate a direct correlation between helicase- and ATPase reduction. F617L and R618L display a disproportional reduction in ATPase and helicase activity, resulting in increased efficiency for F617L and reduced efficiency for R618L, respectively.