Table 1. Estimation of the relative stoichiometry of the soluble T3SS components.

	Relative stoichiometry	Standard deviation	Literature value	References	Spots/bacterium
EGFP-YscD	24.0*	7.8	24	42,43,44,45	8.6
YscV-EGFP	11.2	4.7	9	60	7.2
EGFP-YscK	6.7	2.2			6.8
EGFP-YscQ	27.6	7.2	22±8	39	8.0
EGFP-YscL	14.2	4.7	12	16	7.1
EGFP-YscN	12.2	3.3	6/12	12,38,61	7.2

T3SS, type III secretion system.

Bacteria expressing the denoted fluorescently labelled proteins were imaged and the total fluorescence intensity of each spot of 1,900–3,640 detected spots from 262 to 537 bacteria per strain was used to estimate the relative stoichiometry of the labelled components at the injectisome, based on the reference of YscD, present in 24 subunits per injectisome. The resulting relative stoichiometry and average number of detected spots per bacterium are displayed in bold font.

^*YscD, present in 24 copies, was used as reference for the stoichiometry of the other T3SS components.