Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jun 27;8:15916. doi: 10.1038/ncomms15916

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017, The Author(s)

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(a) Western blot for Beclin-1 and Atg-5 in MCF7 and T47D cells after transfection with Scrambled (Scr), Beclin-1 or Atg5 shRNA. MCF7 and T47D cells with Beclin-1 or Atg5 knocked down were treated with palbociclib for 6 days and subjected to (b) dose–response assay after 6 days of recovery and (c) SA-ß galactosidase measurement. P values calculated in comparison with SCR −1 μM palbociclib. MCF7 and T47D cells were treated with combination of palbociclib and 15 μM HCQ for 6 days. Cells were allowed to recover for 4 or 6 days in drug-free media and subjected to (d) Cell counting, (e) clonogenic assay, (f) cellular ROS measurement and (g) SA-ß galactosidase measurement. (h) Clonogenic assay to measure impact of combined siRNA against CDK4/6 and treatment with 15 μM HCQ for 6 days. MCF7 and T47D cells were treated with 5 μM abemaciclib combined with 15 μM HCQ for 6 days and recovery for 4 or 6 days and subjected to (i) cell counting (j) measurement of total apoptotic cells (early apoptosis: Annexin V+/propidium iodide− and late apoptosis: Annexin V+/propidium iodide+) and (k) clonogenic assay. (l) Clonogenic assay in MCF7 and T47D cells treated with 10 μM spautin-1 or 1 nM bafilomycin A1 (Baf-A1) combined with 1 μM palbociclib for 6 days and recovery for 6 days. (m,n) Cell counting to assess growth of cells treated with 1 μM palbociclib and 7.5 μM Lys-05 (m) or 10 μM Spautin-1 (n) for 6 days and recovery for 4 days. All data represent mean±s.d. from three independent experiments; NS: P>0.05; *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001.