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. 2017 Jun 26;8:15926. doi: 10.1038/ncomms15926

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Copyright © 2017, The Author(s)

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Illustration of genetic alleles used to generate: AdipoTrak (AT)-control (PPARγ^tTA; TRE-H2B-GFP; TRE-Cre); Sox-PPARγ-LOF (Sox2-Cre; PPARγ^f/tTA; TRE-H2B-GFP); AT-PPARγ-LOF (PPARγ^f/tTA; TRE-Cre; TRE-H2B-GFP); AT-PPARγ-GOF (PPARγ^tTA; TRE-H2B-GFP; TRE-PPARγ); and AT-PPARγ-Rescue (PPARγ^f/tTA; TRE-PPARγ; TRE-Cre; TRE-H2B-GFP). Experiments were performed three times on six mice per group. (b) Representative H&E images from mice described in a. (c) Representative GFP images of freshly isolated subcutaneous IGW depots from mice described in a. Scale bar 10 mm. (d) Representative images of CD31 (endothelial marker) and SMA (mural cell marker) and APC-GFP immunostaining from subcutaneous IGW depots from mice described in a. (e) Representative images of SVPs from subcutaneous adipose depots from mice described in a. Locality of APCs was assessed by GFP fluorescence 12 h after isolation. DAPI was used to visual nuclei and cell number (n=9). Scale bars 100 μm.