Figure 2.

Agarose gel electrophoresis of 18S rRNA PCR products of different species of Mucorales after restriction digestion with BmgBI (Lanes 1, 2, & 3), AfIII (Lanes 4, 5, 8, & 9), CspsI (Lanes 6 & 7), XmnI (Lanes 10, 11, 12, & 13), and PpumI (Lanes 14 & 15); Lane 1: Rhizopus spp. (except R. stolonifer); Lanes 2 & 3: undigested PCR products; Lanes 4, 5, 8, & 9: Mucor spp.; Lanes 6 & 7: Rhizopus arrhizus; Lanes 10, 11, 12, & 13: M. circinelloides, or M. racemosus, or M. ramosissimus, or M. plumbeus; Lanes 14 & 15: Rhizomucor spp.; Lane N: negative control; Lane M: 100 bp molecular-size marker