Skip to main content
. Author manuscript; available in PMC: 2017 Jun 29.
Published in final edited form as: Methods Mol Biol. 2017;1527:81–99. doi: 10.1007/978-1-4939-6625-7_7

Fig. 1.

Fig. 1

Scheme for the processing of the precursor protein angiotensinogen to the bioactive peptides angiotensin II (Ang II) and Ang-(1–7). The enzyme renin cleaves angiotensinogen at the N-terminus to form the decapeptide Ang I. Processing of Ang I to the octapeptide Ang II involves angiotensin converting enzyme (ACE) and chymase (CHYM). Subsequent processing to the heptapeptide Ang-(1–7) from Ang II occurs through ACE2 and prolyl oligopeptidase (POP). Note the shared N-terminal sequence among all three peptides but the unique C-terminal sequence that dictates the specificity of C-terminus directed antibodies utilized for radioimmunoassays